University of Rochester
In this project, I observe two kinds of evergreen needles, Arborvitae and Juniper via SEM. HMDS drying and sputter coating are utilized as pretreatments for biological samples. In SEM imaging process, SE2 mode imaging is used to obtain topographical information of samples. Stamata sizes in two evergreen needles are obtained via ImageJ program. The tiny size of stomata on narrow needle leaves facilitate evergreens survive from cold and drought. BSD mode imaging and X-ray Microanalysis work together to study the sample compositions.
This is my first time to have a chance to do a project using SEM. I would like to appreciate Brian’s kind help for the class and my project.