Fast Volumetric Imaging in Brain Tissue

Professor Jerome Mertz, Professor of Biomedical Engineering at Boston University

Presented in-person in Goergen 101 and on Zoom

Zoom Information

Zoom:https://rochester.zoom.us/j/91669460596?pwd=T1VzbEFreVd3NFR5QnlUV09YWlZWZz09
Meeting ID: 916 6946 0596
Passcode: 0330

Abstract

Obtaining high resolution images in scattering tissue has been a long-standing challenge in microscopy. This challenge is exacerbated when high frame rates are required, or when 3D imaging is required over extended volumes. I will present several tools we have developed to address this challenge. These can be applied to a variety of microscopy modalities ranging from multiplane imaging with speckle, to multi-focus confocal imaging with targeted illumination, and finally to multiphoton imaging. Our main applications are in-vivo imaging in the mouse brain, where we demonstrate high contrast kHz-rate imaging of neuronal activity and blood flow deep in the tissue.

Biography

Jerome Mertz received an AB in physics from Princeton University in 1984, and a PhD in quantum optics from UC Santa Barbara and the University of Paris VI in 1991. Following postdoctoral studies at the University of Konstanz and Cornell University, he became a CNRS research director at the Ecole Superieure de Physique et de Chimi Industrielle in Paris. He is currently a professor of Biomedical Engineering at Boston University. His interests are in the development and applications of novel optical microscopy techniques for biological imaging. He is also the author of a textbook titled, "Introduction to Optical Microscopy, 2nd Ed."