Colloquia & Guest Speakers
Fast volumetric light-sheet microscopy
Pablo Loza-Alvarez, The Institute of Photonic Sciences (ICFO), Barcelona, Spain
Friday, February 13, 2015
3 p.m.4 p.m.
Light sheet fluorescence microscopy (LSFM) is a convenient tool for bio-imaging as it efficiently collects the generated fluorescence while at the same time minimizes photobleaching. Recently, the selective plane illumination microscopy (SPIM), being based on an intrinsic 2D illumination, has been put forward as an interesting candidate for fast volumetric imaging. In spite of this, fast SPIM implementations still rely on the movement of the sample or other optical elements to acquire the optical sections. This put constrains when imaging at high speeds, especially when working with living samples as moving artifacts could be adversely affect them. Overcoming this limitation, by scanning only the light-sheet instead, would provide real unperturbed, non-invasive imaging.
Dr. Loza-Alvarez will report on the use of an alternative version of SPIM for fast 4D (3D+time) imaging where only the light sheet is scanned at speeds limited only by the integration time of the camera. This is done without any mechanical scan of the sample. The optical setup is based on the use of a deformable mirror that is placed in the detection path of the SPIM microscope.